The Cherokee National Holiday in Tahlequah celebrates the signing of the Cherokee Nation Constitution in 1839. This annual event is a celebration of Cherokee heritage and cultural awareness. The Cherokee National Holiday attracts visitors from across the United States as well as from around the world. The three-day holiday is full of activities for all ages, from traditional Native American games like cornstalk and blowgun shooting, marbles and stickball to tournaments in sports like basketball and softball.

Many other events will take place during the Cherokee National Holiday, including a parade, children's events, a quilt show and a car show. Vendors will be on hand offering authentic Native American products such as food, artwork, pottery, blankets and other unique items at the Cherokee Artisan Marketplace held on the museum lawn. The highlights of the celebration will be the inter-tribal powwows, held on both Friday night and Saturday night.